Profile search in scMontage can be run by selecting the database to be searched and specifying the query profile.
STEP 1Select database by specifying the organism and platform. Currently, scMontage deals with H. sapiens and M. musculus as organism, and HiSeq 2000/2500, NextSeq 500, MiSeq, and GenomeAnalyzer IIx as platform.
STEP 2Specify genes to be used for calculation according to Gene Ontology (GO).
STEP 3Input a gene expression profile in CM format from user's original data or files via "Data Downloads" on the top page on SHOGoiN database.
STEP 4Select gene assignment resource of the query data (e.g., Ensembl).
STEP 5 (Optional)Set parameters for searching.